9PLW
The structure of O-glycopeptidase BcM60K from Bacteroides caccae in complex with a core 2 glycan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-19 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.835, 64.995, 93.590 |
| Unit cell angles | 90.00, 100.92, 90.00 |
Refinement procedure
| Resolution | 24.790 - 2.190 |
| R-factor | 0.1776 |
| Rwork | 0.175 |
| R-free | 0.22160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.870 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.240 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmeas | 0.106 | 0.525 |
| Rpim | 0.052 | 0.290 |
| Number of reflections | 28358 | 1401 |
| <I/σ(I)> | 13.1 | 2.2 |
| Completeness [%] | 99.6 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.919 | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M Bis-Tris pH6.0, 20% PEG3350 |
