9PLV

X-ray crystal structure of the beta-carotene oxygenase like g (BCOLg) from Lancelet floridae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	95
Detector technology	PIXEL
Collection date	2024-12-08
Detector	DECTRIS EIGER2 R 4M
Wavelength(s)	1.54178
Spacegroup name	C 1 2 1
Unit cell lengths	98.460, 139.910, 93.760
Unit cell angles	90.00, 98.26, 90.00

Refinement procedure

Resolution	24.560 - 2.400
R-factor	0.1774
Rwork	0.175
R-free	0.22420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.021
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.850	2.460
High resolution limit [Å]	2.400	2.400
Rmeas	0.101	0.393
Number of reflections	48073	3240
<I/σ(I)>	11.98	2.82
Completeness [%]	98.1	89.6
Redundancy	4.25	3.04
CC(1/2)	0.996	0.847

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	Condition 22 of Crystal Screen (Hampton Research, HR2-110) supplemented with 5% ethylene glycol.