9PLU

[20-7B G|T] 20 bp tensegrity triangle that propagates via blunt-end stacking with G stacking on T at the interface

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.00743
Spacegroup name	I 41 3 2
Unit cell lengths	166.197, 166.197, 166.197
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.550 - 5.670
R-factor	0.1728
Rwork	0.171
R-free	0.19550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.871
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	67.850	67.850	6.220
High resolution limit [Å]	5.670	11.055	5.674
Number of reflections	1206	202	204
<I/σ(I)>	21.2
Completeness [%]	92.3
Redundancy	51.7
CC(1/2)	1.000	1.000	0.672

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate