9PLC
JLM3 left-handed, parallel G-quadruplex in P65 2 2 Spacegroup
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 196 |
| Detector technology | PIXEL |
| Collection date | 2025-04-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979330 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 30.889, 30.889, 114.068 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.750 - 1.540 |
| R-factor | 0.1695 |
| Rwork | 0.168 |
| R-free | 0.20420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.183 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHENIX (1.21.2_5419) |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.044 | 1.565 |
| High resolution limit [Å] | 1.538 | 1.538 |
| Rmerge | 0.074 | 2.965 |
| Rmeas | 0.075 | 3.005 |
| Rpim | 0.013 | 0.485 |
| Number of reflections | 5136 | 258 |
| <I/σ(I)> | 18.9 | 1.3 |
| Completeness [%] | 95.8 | 100 |
| Redundancy | 34.4 | |
| CC(1/2) | 0.999 | 0.510 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 285 | 0.08 M Strontium chloride, 0.04 M Sodium cacodylate 6.0, 35% w/v MPD, 0.012 M Spermine tetrahydrochloride |
