9PL8

The structure of O-glycopeptidase BcM60C from Bacteroides caccae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-13
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	118.605, 118.605, 90.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.830 - 1.550
R-factor	0.1861
Rwork	0.185
R-free	0.20990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.052
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.580
High resolution limit [Å]	1.550	1.550
Rmeas	0.089	0.569
Rpim	0.026	0.263
Number of reflections	93992	4620
<I/σ(I)>	27.5	2.4
Completeness [%]	99.8
Redundancy	7.8
CC(1/2)	0.997	0.855

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M (NH4)2 SO4, 0.1M Na citrate pH5.6, 21% PEG4000