9PL7
The structure of O-glycopeptidase BcM60K from Bacteroides caccae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-31 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.850, 64.892, 93.415 |
| Unit cell angles | 90.00, 100.81, 90.00 |
Refinement procedure
| Resolution | 29.820 - 2.100 |
| R-factor | 0.2185 |
| Rwork | 0.216 |
| R-free | 0.25470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.416 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.092 | 0.288 |
| Rpim | 0.047 | 0.199 |
| Number of reflections | 31868 | 1503 |
| <I/σ(I)> | 11.6 | 2.5 |
| Completeness [%] | 98.8 | |
| Redundancy | 2.9 | |
| CC(1/2) | 0.983 | 0.894 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M Bis-Tris pH6.0, 20% PEG3350 |
