9PL2

Crystal structure of a SnoaL-like domain-containing protein from Mycobacterium ulcerans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 19-ID
Synchrotron site	NSLS-II
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-05
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.9786
Spacegroup name	P 21 21 2
Unit cell lengths	57.632, 131.167, 33.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.290 - 1.370
R-factor	0.1541
Rwork	0.152
R-free	0.18970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.975
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((2.0_5750: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.720	1.390
High resolution limit [Å]	1.370	1.370
Rmerge	0.064	1.079
Rmeas	0.067	1.158
Rpim	0.019	0.408
Total number of observations	656684	18480
Number of reflections	53934	2428
<I/σ(I)>	18	1.6
Completeness [%]	99.5
Redundancy	12.2	7.6
CC(1/2)	0.999	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.8	291	8% (w/v) PEG 4K, 0.1M Cit pH 3.8, 10mM potassium dihydrogen phosphate. MyulA.17060.a.A1.PB00143 at 15 mg/mL. plate Liu-S-182 C1 Puck: PSL0114, Cryo: 75% crystallant + 25% PEG 200.