9PKT
The structure of O-glycopeptidase BcM60B from Bacteroides caccae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-14 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.073, 109.404, 96.746 |
| Unit cell angles | 90.00, 94.81, 90.00 |
Refinement procedure
| Resolution | 29.780 - 1.800 |
| R-factor | 0.1652 |
| Rwork | 0.164 |
| R-free | 0.19340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.957 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmeas | 0.070 | 0.251 |
| Rpim | 0.034 | 0.162 |
| Number of reflections | 92252 | 4571 |
| <I/σ(I)> | 19.4 | 4.2 |
| Completeness [%] | 100.0 | 99.6 |
| Redundancy | 4 | |
| CC(1/2) | 0.997 | 0.906 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 4% Tascimate pH4.0, 15% PEG3350, Cryo 20% EG |
