9PKS
The structure of O-glycopeptidase BcM60F from Bacteroides caccae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-10 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.252, 96.724, 85.870 |
| Unit cell angles | 90.00, 114.56, 90.00 |
Refinement procedure
| Resolution | 29.800 - 1.800 |
| R-factor | 0.1541 |
| Rwork | 0.152 |
| R-free | 0.18380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.902 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmeas | 0.055 | 0.166 |
| Rpim | 0.025 | 0.100 |
| Number of reflections | 107678 | 5256 |
| <I/σ(I)> | 27.4 | |
| Completeness [%] | 99.0 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.998 | 0.959 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M Na citrate tribasic dihydrate pH5.6, 20% 2-Propanol, 20% PEG4K |
