9PKQ
Structure of the cytoplasmic domain of unliganded human Tom70, open and closed conformations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-20 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.448, 89.361, 105.775 |
| Unit cell angles | 90.00, 104.86, 90.00 |
Refinement procedure
| Resolution | 102.240 - 2.000 |
| R-factor | 0.2295 |
| Rwork | 0.228 |
| R-free | 0.25990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.915 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 102.240 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.048 | 1.006 |
| Number of reflections | 77515 | 2774 |
| <I/σ(I)> | 12.2 | 0.8 |
| Completeness [%] | 97.4 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1M MES (pH 6.5), 0.1 M sodium acetate, and 30% PEG2000 MME |
