9PIU
Crystal structure of a synthetic Fab (2C) in complex with the FRB domain of mTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.860, 73.441, 91.971 |
| Unit cell angles | 90.00, 102.78, 90.00 |
Refinement procedure
| Resolution | 33.400 - 2.180 |
| R-factor | 0.1917 |
| Rwork | 0.190 |
| R-free | 0.23290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.546 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2-5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.400 | 2.260 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Number of reflections | 31815 | 2879 |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 99.9 | |
| Redundancy | 2 | |
| CC(1/2) | 0.984 | 0.441 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2 M sodium tartrate dibasic, 20% PEG 3350 |
