9PI6
Single stranded DNA-binding protein (ICP8) from Herpes simplex virus-1, apo form.Mutations: K166A, E167A, C254S, C455S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979300 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.348, 139.779, 98.869 |
| Unit cell angles | 90.00, 111.09, 90.00 |
Refinement procedure
| Resolution | 92.417 - 2.750 |
| Rwork | 0.177 |
| R-free | 0.26090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.693 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.420 | 2.830 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.107 | 0.495 |
| Rmeas | 0.151 | 0.703 |
| Rpim | 0.107 | 0.497 |
| Number of reflections | 59491 | 4620 |
| <I/σ(I)> | 7.2 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 2 | 2 |
| CC(1/2) | 0.979 | 0.545 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2% PEG 8,000, 100mM Hepes, pH 7.0, 40mM MgCl2, 2mM DTT, 1mM Spermine |
