9PI3

Single stranded DNA-binding protein (ICP8) from Herpes simplex virus-1, apo form. Mutations: C254S, C455S, K769A, E770A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920085
Spacegroup name	P 1 21 1
Unit cell lengths	89.990, 140.200, 98.800
Unit cell angles	90.00, 111.06, 90.00

Refinement procedure

Resolution	92.200 - 2.750
Rwork	0.184
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.614
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.200	2.850
High resolution limit [Å]	2.750	2.750
Rmerge	0.238	1.030
Number of reflections	59439	4618
<I/σ(I)>	7.5	2.5
Completeness [%]	100.0
Redundancy	6.9
CC(1/2)	0.983	0.735

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	2% PEG 8,000, 50mM HEPES, pH 7.5, 40mM MgCl2, 2mM DTT, 1mM Spermine