9PHZ

Crystal structure of the Spo0B-Spo0A complex from Bacillus subtilis (crystal form I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97622
Spacegroup name	P 21 21 21
Unit cell lengths	76.361, 125.504, 149.472
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.890 - 2.049
R-factor	0.2235
Rwork	0.222
R-free	0.24430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.850
Data reduction software	DIALS (1.11.1)
Data scaling software	Aimless (0.7.2)
Phasing software	PHASER
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.360	2.060
High resolution limit [Å]	2.050	2.050
Rmeas	0.071	1.506
Rpim	0.028	0.667
Number of reflections	90767	4368
<I/σ(I)>	11.15	1.2
Completeness [%]	100.0	97.7
Redundancy	6.2
CC(1/2)	0.999	0.466

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M sodium fluoride, 20% w/v PEG3350