9PHM

[A4J-R] Asymmetric tensegrity triangle formed via toehold mediated displacement of the center strand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-21
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920174
Spacegroup name	P 1
Unit cell lengths	67.486, 68.579, 70.075
Unit cell angles	101.82, 97.76, 101.02

Refinement procedure

Resolution	65.400 - 6.550
R-factor	0.1631
Rwork	0.161
R-free	0.17270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.150
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	67.453	67.453	7.874
High resolution limit [Å]	6.550	14.372	6.553
Number of reflections	1497	214	214
<I/σ(I)>	3.3
Completeness [%]	80.5
Redundancy	1.9
CC(1/2)	0.647	0.577	0.428

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate