9PGP

[S1M] Tensegrity triangle with a 1 nucleotide single stranded region on one side

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920208
Spacegroup name	P 1
Unit cell lengths	68.616, 68.633, 68.666
Unit cell angles	100.66, 103.13, 101.97

Refinement procedure

Resolution	41.770 - 8.020
R-factor	0.1732
Rwork	0.172
R-free	0.20880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.963
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.119	65.119	8.479
High resolution limit [Å]	8.017	14.576	8.017
Number of reflections	1223	204	204
<I/σ(I)>	13.3
Completeness [%]	99.8
Redundancy	2.3
CC(1/2)	0.702	0.575	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate