9PFN

Structure of POU2F3 POU domains bound to coactivator OCA-T2 and DNA (2.8 angstrom resolution)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-30
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.979493
Spacegroup name	P 1 21 1
Unit cell lengths	81.165, 36.022, 88.598
Unit cell angles	90.00, 90.08, 90.00

Refinement procedure

Resolution	38.910 - 2.790
R-factor	0.2385
Rwork	0.236
R-free	0.29560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.642
Data reduction software	XDS (February 5, 2021)
Data scaling software	pointless (1.12.10)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.910	2.940
High resolution limit [Å]	2.790	2.790
Rmerge	0.099	0.759
Rmeas	0.119	0.902
Rpim	0.064	0.483
Total number of observations	44150	5884
Number of reflections	13089	1765
<I/σ(I)>	6.8	1.4
Completeness [%]	99.2
Redundancy	3.4	3.3
CC(1/2)	0.990	0.719

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	291	0.1 M MIB (2:3:3 molar ratio of malonate:imidazole:boric acid), pH 4.0, 25% PEG-1500