9PFD

RMI1-RMI2 bound to synthetic peptide P4Ser

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953730
Spacegroup name	P 41 21 2
Unit cell lengths	117.541, 117.541, 53.475
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.722 - 2.008
Rwork	0.181
R-free	0.22410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	2.442
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.772	2.060
High resolution limit [Å]	2.008	2.010
Number of reflections	25632	1866
<I/σ(I)>	35.2
Completeness [%]	100.0
Redundancy	26.3
CC(1/2)	1.000	0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	1.8 M sodium phosphate monobasic monohydrate, potassium phosphate dibasic, pH 5.0