9PFA

Crystal structure of the glycosyltransferase SvFucT from Saponaria vaccaria

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-01
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	50.564, 110.730, 170.548
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.480 - 1.740
R-factor	0.1756
Rwork	0.175
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.025
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.480	1.800
High resolution limit [Å]	1.740	1.740
Number of reflections	91222	7425
<I/σ(I)>	11.8
Completeness [%]	100.0
Redundancy	7.3
CC(1/2)	0.999	0.335

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M magnesium chloride, 0.1 M Bis-Tris pH 6.5 25 % (w/v) PEG 3,350