9PF9

X-ray crystal structure of ATX-350-2 Fab bound to Epstein-Barr virus glycoprotein 350

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000040
Spacegroup name	P 43 21 2
Unit cell lengths	116.675, 116.675, 200.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.260 - 3.930
R-factor	0.2435
Rwork	0.242
R-free	0.28190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.588
Data reduction software	XDS
Data scaling software	Aimless (0.8.2)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (2.0_5793)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.260	4.320
High resolution limit [Å]	3.928	3.930
Rmerge	0.328	1.695
Rmeas	0.341	1.767
Rpim	0.129	0.689
Number of reflections	12880	3126
<I/σ(I)>	9.7	1.8
Completeness [%]	99.7
Redundancy	12.7
CC(1/2)	0.996	0.689

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.1 M Sodium Citrate:HCl pH 5. 3.15 M Ammonium Sulfate