9PF8

[A1J] Asymmetric tensegrity triangle with a 1 nucleotide single stranded region adjacent to the junction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920208
Spacegroup name	P 1
Unit cell lengths	68.390, 68.450, 68.550
Unit cell angles	98.48, 95.51, 108.60

Refinement procedure

Resolution	51.250 - 6.100
R-factor	0.2823
Rwork	0.282
R-free	0.29660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.940
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	67.007	67.007	7.129
High resolution limit [Å]	6.100	14.420	6.104
Number of reflections	1829	203	203
<I/σ(I)>	3.9
Completeness [%]	83.0
Redundancy	2.2
CC(1/2)	0.627	0.513	0.228

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate