9PF7

[A1M] Asymmetric tensegrity triangle with a 1 nucleotide single stranded region on one side

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-21
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920174
Spacegroup name	P 1
Unit cell lengths	66.715, 68.534, 68.698
Unit cell angles	100.89, 98.91, 102.74

Refinement procedure

Resolution	65.030 - 4.590
R-factor	0.225
Rwork	0.224
R-free	0.24350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.904
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	66.026	66.026	5.252
High resolution limit [Å]	4.587	14.520	4.587
Number of reflections	3877	204	204
<I/σ(I)>	2.3
Completeness [%]	86.1
Redundancy	2.3
CC(1/2)	0.774	0.760	0.153

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate