9PF6

[A4M] Asymmetric tensegrity triangle with a 4 nucleotide single stranded region on one side

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97934
Spacegroup name	P 1
Unit cell lengths	68.847, 68.850, 69.175
Unit cell angles	105.48, 96.06, 95.90

Refinement procedure

Resolution	65.730 - 6.830
R-factor	0.2015
Rwork	0.200
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.055
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	67.819	67.819	8.002
High resolution limit [Å]	6.830	14.419	6.832
Number of reflections	1439	206	206
<I/σ(I)>	5.7
Completeness [%]	79.7
Redundancy	1.9
CC(1/2)	0.997	0.996	0.608

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate