9PF5

[A4J] Asymmetric tensegrity triangle with a 4 nucleotide single stranded region adjacent to the junction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-05-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.979338
Spacegroup name	P 1
Unit cell lengths	67.936, 68.202, 68.149
Unit cell angles	88.29, 89.97, 65.89

Refinement procedure

Resolution	46.150 - 5.620
R-factor	0.2534
Rwork	0.252
R-free	0.28470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.879
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.219	62.219	6.649
High resolution limit [Å]	5.618	14.057	5.618
Number of reflections	2053	205	205
<I/σ(I)>	5.1
Completeness [%]	80.6
Redundancy	2.3
CC(1/2)	0.988	0.997	0.468

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate