9PDZ

Crystal structure of a Spermidine binding protein isolated from Synechococcus CC9311

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I23
Synchrotron site	Diamond
Beamline	I23
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-06-26
Detector	DECTRIS PILATUS 12M
Wavelength(s)	2.7552
Spacegroup name	P 1 21 1
Unit cell lengths	39.080, 65.980, 64.370
Unit cell angles	90.00, 98.63, 90.00

Refinement procedure

Resolution	45.847 - 1.800
Rwork	0.183
R-free	0.21940
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	2.343
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.105))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.847	1.830
High resolution limit [Å]	1.796	1.800
Number of reflections	27220	929
<I/σ(I)>	19.6
Completeness [%]	90.3
Redundancy	11.4
CC(1/2)	0.999	0.611

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	20% (w/v) PEG 8000, 100 mM HEPES/ Sodium hydroxide pH 7.5
1	VAPOR DIFFUSION, SITTING DROP		295	20% (w/v) PEG 8000, 100 mM HEPES/ Sodium hydroxide pH 7.5
1	VAPOR DIFFUSION, SITTING DROP		295	20% (w/v) PEG 8000, 100 mM HEPES/ Sodium hydroxide pH 7.5