9PCQ

Phosphorylation of a Conserved Aspartate at the Eukaryotic Elongation Factor 2 Kinase Catalytic Site

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920099
Spacegroup name	P 21 21 21
Unit cell lengths	60.464, 88.632, 154.872
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.460 - 2.300
R-factor	0.2129
Rwork	0.211
R-free	0.24290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.663
Data reduction software	autoPROC
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.436	2.339
High resolution limit [Å]	2.299	2.299
Rmeas	0.324
Rpim	0.088
Number of reflections	37754	1877
<I/σ(I)>	6.7
Completeness [%]	99.7	98.7
Redundancy	13.5	14.3
CC(1/2)	0.995	0.373

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298.15	Protein was mixed 1/1 with 100 mM BisTrisPropane, 80 mM NaF, 20% w/v PEG3350