9PCA

HUMAN PRMT5:MEP50 COMPLEX IN COMPLEX WITH LIGAND 18

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-13
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.8856
Spacegroup name	I 2 2 2
Unit cell lengths	102.807, 139.139, 179.354
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	109.940 - 2.200
R-factor	0.22612
Rwork	0.224
R-free	0.26621
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	1.302
Data reduction software	autoPROC (1.1.7)
Data scaling software	autoPROC (Version 1.1.7)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	109.940	2.541
High resolution limit [Å]	2.197	2.197
Rpim	0.062	0.547
Number of reflections	34528	1726
<I/σ(I)>	7.7	1.6
Completeness [%]	52.6	7.5
Redundancy	12.9	12.4
CC(1/2)	0.983	0.587

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	0.27 M (NH4)2SO4; 0.05 M MES pH 6.75; 23.0% w/v PEG 3350 PROTEIN SOLUTION : 2 mM DTT, 10 % Glycerol, 10 mM HEPES-NaOH pH 7.5, 150 mM NaCl