9PAI
CLEAVED SUBSTRATE VARIANT OF PLASMINOGEN ACTIVATOR INHIBITOR-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 287 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-09-15 |
| Detector | SIEMENS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.860, 47.520, 62.730 |
| Unit cell angles | 90.00, 113.95, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.700 |
| Rwork | 0.181 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | LATENT PAI-1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.090 | |
| Number of reflections | 9914 | |
| <I/σ(I)> | 9.92 | 1.6 |
| Completeness [%] | 71.0 | 61 |
| Redundancy | 2.5 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 19% (W/V) PEG 4000, 100 MM TRIS PH 8.5 |
