9P8W

Anti-phage dGTPase from Shewanella putrefaciens CN-32

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979200
Spacegroup name	P 63 2 2
Unit cell lengths	121.718, 121.718, 151.483
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	75.740 - 2.680
R-factor	0.211
Rwork	0.208
R-free	0.26580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.488
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	105.410	2.810
High resolution limit [Å]	2.680	2.680
Rmerge	0.378	2.970
Rmeas	0.387
Rpim	0.084	0.658
Number of reflections	19233	2502
<I/σ(I)>	11.9	1.9
Completeness [%]	99.9	100
Redundancy	39	40.1
CC(1/2)	0.853	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.2 M magnesium chloride, 0.1 M HEPES-NaOH, pH 7.5, 30% PEG400