9P6D
Crystal Structure of DUF4097 domain-Containing Protein from Clostridium difficile Strain 630
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-25 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.814, 73.033, 120.136 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.790 - 2.000 |
| R-factor | 0.20341 |
| Rwork | 0.201 |
| R-free | 0.24956 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.390 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.127 | |
| Rmeas | 0.140 | |
| Rpim | 0.057 | 0.478 |
| Number of reflections | 36987 | 1845 |
| <I/σ(I)> | 14.8 | 1.94 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.9 | 6.1 |
| CC(1/2) | 0.996 | 0.703 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | Protein: 7.0 mg/ml, 0.5M Sodium chloride, 0.01M Tris pH 8.3; Screen: PEGs II (B6), 0.1M HEPES (pH 7.5), 20% (w/v) PEG1500 Cryo: Reservoir |
