9P5Y

Crystal structure of hen egg white lysozyme at 100 Kelvin with Vaseline

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER TURBO X-RAY SOURCE
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-01
Detector	Bruker PHOTON II
Wavelength(s)	1.54184
Spacegroup name	P 43 21 2
Unit cell lengths	77.343, 77.343, 37.399
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.458 - 1.497
Rwork	0.178
R-free	0.20300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.786
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.458	24.458
High resolution limit [Å]	1.497	1.497
Number of reflections	18783	18783
<I/σ(I)>	19
Completeness [%]	99.0
Redundancy	1
CC(1/2)	0.990	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	295	1.2 M NaCl and 100 mM AcONa pH 4.6