9P4H

Streptomyces thermoviolaceus ClpC2 C-terminal domain with bound Cyclomarin A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	.97934
Spacegroup name	C 1 2 1
Unit cell lengths	50.513, 44.061, 70.350
Unit cell angles	90.00, 109.36, 90.00

Refinement procedure

Resolution	33.190 - 1.550
R-factor	0.1493
Rwork	0.148
R-free	0.17410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.614
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.580
High resolution limit [Å]	1.550	1.550
Number of reflections	35083	927
<I/σ(I)>	26.78
Completeness [%]	98.8
Redundancy	4.7
CC(1/2)	0.998	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M Bis-tris, pH 5.75, 29% PEG3350, 1 mM Cyclomarin A