9P4G

Crystal Structure of Flagellar Assembly Protein FliW from Campylobacter jejuni subsp. jejuni 81-176-DRH212

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2020-10-05
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	43.954, 70.912, 100.937
Unit cell angles	90.00, 94.20, 90.00

Refinement procedure

Resolution	29.110 - 2.950
R-factor	0.25466
Rwork	0.252
R-free	0.29489
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.884
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.000
High resolution limit [Å]	2.950	2.950
Rmerge	0.202
Rpim	0.078	0.643
Number of reflections	13319	654
<I/σ(I)>	11.8	1.6
Completeness [%]	100.0	100
Redundancy	7.6	7.7
CC(1/2)	0.993	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	Protein: 7.3 mg/ml, 0.01M Tris pH 8.3; Screen: ComPAS (G2), 0.1M HEPES pH 7.5, 0.7M Sodium citrate; Cryo: 20% Glycerole in reservoir solution.