9P41

Crystal structure of endoglucanase Cel5A from Rhizobium sp. C1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2022-08-12
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	C 2 2 21
Unit cell lengths	76.477, 117.870, 86.343
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.680 - 1.560
R-factor	0.171
Rwork	0.170
R-free	0.19180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.880
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: 000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.680	1.616
High resolution limit [Å]	1.560	1.560
Rmerge	0.015	0.220
Rmeas	0.021	0.310
Rpim	0.015	0.220
Number of reflections	55337	5135
<I/σ(I)>	15.21	2.01
Completeness [%]	99.3	93.29
Redundancy	2	2
CC(1/2)	1.000	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	291.15	0.1 M Citrate, pH 5.0, 20% w/v PEG 6000