9P40
MEKK1 SWIM and RING Domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 65 |
| Unit cell lengths | 119.832, 119.832, 56.581 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.171 - 3.050 |
| Rwork | 0.179 |
| R-free | 0.22030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.173 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.88)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.171 | 3.260 |
| High resolution limit [Å] | 2.950 | 3.050 |
| Rmerge | 0.203 | 1.048 |
| Rmeas | 0.222 | 1.151 |
| Rpim | 0.091 | 0.469 |
| Number of reflections | 8923 | 1600 |
| <I/σ(I)> | 7.5 | 1.7 |
| Completeness [%] | 99.0 | 99.3 |
| Redundancy | 5.8 | 5.8 |
| CC(1/2) | 0.990 | 0.710 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 291.15 | 0.04 M NaH2PO4 and 0.96 M K2HPO4 |
