9P3K

Crystal Structure of Xyloglucan Xylosyltransferase 2 and Xyloglucan Xylosyltransferase 5 Heterodimer Bound to UDP and Cellohexaose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2024-04-17
Detector	RDI CMOS_8M
Wavelength(s)	1.000
Spacegroup name	P 1
Unit cell lengths	77.715, 111.851, 115.670
Unit cell angles	89.34, 70.44, 80.92

Refinement procedure

Resolution	48.360 - 3.200
R-factor	0.2311
Rwork	0.229
R-free	0.28990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.566
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.180	3.020
High resolution limit [Å]	2.960	2.960
Rmerge	0.237	2.058
Rmeas	0.278	2.428
Rpim	0.144	1.274
Total number of observations	265692	14812
Number of reflections	73011	4275
<I/σ(I)>	5	0.6
Completeness [%]	96.7
Redundancy	3.6	3.5
CC(1/2)	0.987	0.347

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20-24% PEG 3350, 0.1 M Lithium Sulfate, and 0.1 M Bis-Tris propane or HEPES