9P3J

Crystal Structure of Xyloglucan Xylosyltransferase 2 and Xyloglucan Xylosyltransferase 5 Heterodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2023-11-19
Detector	RDI CMOS_8M
Wavelength(s)	1.0001
Spacegroup name	P 1
Unit cell lengths	80.492, 112.863, 116.607
Unit cell angles	84.00, 69.81, 79.20

Refinement procedure

Resolution	49.080 - 3.200
R-factor	0.2224
Rwork	0.220
R-free	0.26690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.553
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.080	3.020
High resolution limit [Å]	2.960	2.960
Rmerge	0.350	1.906
Rmeas	0.412	2.238
Rpim	0.216	1.165
Total number of observations	276925	16191
Number of reflections	78477	4490
<I/σ(I)>	2.6	0.6
Completeness [%]	99.6
Redundancy	3.5	3.6
CC(1/2)	0.944	0.443

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	27% polyethylene glycol 3350 (PEG 3350), .2 M Lithium Sulfate, and .1 M Bis-Tris Propane pH 7.0