9P3H

Crystal Structure of Conserved Hypothetical Protein from Stenotrophomonas maltophilia (strain K279a)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.12706
Spacegroup name	P 1 21 1
Unit cell lengths	62.399, 144.741, 79.038
Unit cell angles	90.00, 113.16, 90.00

Refinement procedure

Resolution	30.000 - 1.850
R-factor	0.19359
Rwork	0.192
R-free	0.21491
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	1.216
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.880
High resolution limit [Å]	1.850	1.850
Rmerge	0.117
Rpim	0.047	0.491
Number of reflections	107367	5337
<I/σ(I)>	16.9	2.2
Completeness [%]	98.0	97.6
Redundancy	6.8	6.7
CC(1/2)	0.990	0.703

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	8.9 mM protein, 0.5 M sodium chloride, 0.01 M Tris-HCl, pH 8.3 against PEG's II (F3) [0.1 M HEPES, pH 7.5, 20% w/v PEG4000, 10% w/v isopropanol], cryoprotectant: 0.1 M HEPES, pH 7.5, 20% w/v PEG4000, 10% w/v isopropanol