9P3G
Structure of M. thermoresistible Rv3035
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-01-22 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 69.693, 69.693, 327.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.400 - 1.900 |
| R-factor | 0.1794 |
| Rwork | 0.176 |
| R-free | 0.21360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.851 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.400 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.192 | 3.020 |
| Rmeas | 0.203 | 3.185 |
| Rpim | 0.064 | 1.006 |
| Number of reflections | 65337 | 4124 |
| <I/σ(I)> | 11.7 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 18.5 | 18.8 |
| CC(1/2) | 0.999 | 0.867 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M sodium chloride, 0.1 M HEPES, pH 7.5, and 1.6 M ammonium sulfate |
