9P3E
LC3B bound to BLM peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2023-06-08 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 66.090, 46.740, 45.480 |
| Unit cell angles | 90.00, 102.20, 90.00 |
Refinement procedure
| Resolution | 23.840 - 2.000 |
| R-factor | 0.2241 |
| Rwork | 0.220 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.509 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 23.840 | 23.840 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.089 | 0.035 | 0.894 |
| Rmeas | 0.098 | 0.039 | 1.093 |
| Number of reflections | 9065 | 114 | 555 |
| <I/σ(I)> | 12.12 | ||
| Completeness [%] | 97.6 | ||
| Redundancy | 5.4 | ||
| CC(1/2) | 0.998 | 0.999 | 0.289 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M HEPES, pH 7.3, 30% w/v PEG 3350, and 0.32 MgCl2 |
