9P39

Streptomyces thermoviolaceus ClpC2 C-terminal domain with bound phosphoarginine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE 7B2
Synchrotron site	CHESS
Beamline	7B2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9686
Spacegroup name	P 61
Unit cell lengths	74.084, 74.084, 50.688
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.770 - 1.300
R-factor	0.1689
Rwork	0.168
R-free	0.18970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.087
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.320
High resolution limit [Å]	1.300	1.300
Number of reflections	38875	1911
<I/σ(I)>	69.38
Completeness [%]	99.8
Redundancy	17.2
CC(1/2)	1.000	0.741

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M Bis-tris, pH 5.5, 1 mM pArg, and 2 M ammonium sulfate