9P34
Crystal structure of the Bromo domain 1 in human Bromodomain Containing Protein 4 with a compound
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-29 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92019 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.031, 48.261, 61.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.530 - 0.990 |
| R-factor | 0.1287 |
| Rwork | 0.128 |
| R-free | 0.14970 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.171 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.530 | 1.009 |
| High resolution limit [Å] | 0.990 | 0.992 |
| Rmerge | 0.045 | 0.440 |
| Rmeas | 0.051 | 0.622 |
| Rpim | 0.023 | 0.440 |
| Total number of observations | 236 | |
| Number of reflections | 58095 | 230 |
| <I/σ(I)> | 19.3 | 1.6 |
| Completeness [%] | 79.9 | |
| Redundancy | 4.3 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.2M NaNO3, 20% PEG3350, 10% Ethylene glycol, pH 7.5 |
