9P1R

Crystal structure of TCZ9 from Streptomyces

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-30
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00
Spacegroup name	P 61 2 2
Unit cell lengths	94.937, 94.937, 240.772
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.570 - 1.560
R-factor	0.1931
Rwork	0.192
R-free	0.21520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.868
Data reduction software	XDS (20230630)
Data scaling software	Aimless (0.8.2)
Phasing software	PHENIX ((1.18.2_3874: ???))
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.570	1.590
High resolution limit [Å]	1.560	1.560
Number of reflections	90005	3760
<I/σ(I)>	26.4	0.9
Completeness [%]	98.2	85.7
Redundancy	18.7
CC(1/2)	1.000	0.304

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	293	0.1 M HEPES, pH 7.2, 0.1 M calcium acetate, 19% PEG3350