9P1Q
Crystal structure of Ube2E3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-11-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.560, 62.820, 51.570 |
| Unit cell angles | 90.00, 95.46, 90.00 |
Refinement procedure
| Resolution | 25.681 - 2.150 |
| Rwork | 0.174 |
| R-free | 0.24610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.779 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.681 | 2.150 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.018 | 0.046 |
| Rmeas | 0.026 | 0.065 |
| Rpim | 0.018 | 0.046 |
| Number of reflections | 17482 | 2254 |
| <I/σ(I)> | 13842.9 | 13.3 |
| Completeness [%] | 95.6 | 81.5 |
| Redundancy | 1.7 | 1.53 |
| CC(1/2) | 0.999 | 0.993 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.8 M LiCl, 0.1 M Tris pH 8.5, 32%(w/v) PEG 4000 |
