9P1P

Crystal structure of the apo form of CLZ9 from Streptomyces

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97
Spacegroup name	P 41 21 2
Unit cell lengths	101.378, 101.378, 157.074
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.210 - 1.950
R-factor	0.2283
Rwork	0.227
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.211
Data reduction software	XDS (20230630)
Data scaling software	Aimless (0.8.2)
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.190	1.980
High resolution limit [Å]	1.950	1.950
Number of reflections	60140	2924
<I/σ(I)>	18	0.7
Completeness [%]	100.0	99.6
Redundancy	13.5
CC(1/2)	1.000	0.349

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	293	0.1 M HEPES, pH 7.3, 0.1 M calcium acetate, 15% PEG3350