9P10

Crystal structure of hen egg white lysozyme at 100 Kelvin with Apiezon N (Triplicate)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER TURBO X-RAY SOURCE
Temperature [K]	100
Detector technology	CMOS
Collection date	2023-11-01
Detector	BRUKER PHOTON 100
Wavelength(s)	1.54184
Spacegroup name	P 43 21 2
Unit cell lengths	78.313, 78.313, 37.382
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.558 - 1.496
Rwork	0.216
R-free	0.24920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.850
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.558	24.610
High resolution limit [Å]	1.496	1.500
Number of reflections	19312	1742
<I/σ(I)>	17.53
Completeness [%]	99.3
Redundancy	10
CC(1/2)	0.880	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	295	1.2 M sodium chloride, 100 mM sodium acetate, pH 4.6