Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-08-07 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.345, 41.071, 71.860 |
| Unit cell angles | 90.00, 104.25, 90.00 |
Refinement procedure
| Resolution | 19.900 - 1.880 |
| R-factor | 0.1647 |
| Rwork | 0.163 |
| R-free | 0.18950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.142 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 1.910 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmeas | 0.393 | |
| Rpim | 0.028 | 0.203 |
| Number of reflections | 19252 | 950 |
| <I/σ(I)> | 30.9 | 4.03 |
| Completeness [%] | 97.6 | 97.1 |
| Redundancy | 3.3 | 3.2 |
| CC(1/2) | 0.866 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 293 | 50 mM Tris HCl pH 7.8, 1.6 M Sodium Citrate |
