9P0F

Crystal Structure of the C-terminal Cytoplasmic Domain of nsp4 from SARS-CoV-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.12723
Spacegroup name	C 2 2 21
Unit cell lengths	72.691, 125.541, 89.862
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.690 - 2.350
R-factor	0.2184
Rwork	0.216
R-free	0.25870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.378
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.390
High resolution limit [Å]	2.350	2.350
Rmerge	0.128
Rmeas	0.132
Rpim	0.035	0.562
Number of reflections	17527	832
<I/σ(I)>	27.1	1.8
Completeness [%]	100.0	100
Redundancy	14.9	15.2
CC(1/2)	1.000	0.926

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	Protein: 2.8 mg/ml, 0.15M Sodium chloride, 0.01M Tris pH 8.3; Screen: ComPAS (H8), 0.2M Sodium acetate, 0.1M MES (pH 6.5), 2.0M Sodium chloride; Cryo: 4.0M Sodium formate.