9P0B
Structure of human Sec23a/Sec24a/Sec22b bound to CPD15
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-02 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.795, 96.389, 129.342 |
| Unit cell angles | 90.00, 90.09, 90.00 |
Refinement procedure
| Resolution | 129.340 - 2.530 |
| R-factor | 0.21983 |
| Rwork | 0.218 |
| R-free | 0.28753 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.573 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 129.340 | 2.600 |
| High resolution limit [Å] | 2.530 | 2.530 |
| Rmerge | 0.074 | 0.450 |
| Number of reflections | 57992 | 4061 |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 99.2 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 50 mM Tris, pH 7.8, 0.3 M NaAC, 15% PEG4000 |
