9P05
Structure of human Sec23a/Sec24a/Sec22b bound to CPD11
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-02 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 0.978 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.154, 97.657, 128.772 |
| Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
| Resolution | 64.390 - 2.310 |
| R-factor | 0.2332 |
| Rwork | 0.232 |
| R-free | 0.28590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.152 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.390 | 2.370 |
| High resolution limit [Å] | 2.300 | 2.310 |
| Rmerge | 0.960 | 0.374 |
| Number of reflections | 76146 | 3758 |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 95.0 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 50 mM Tris, pH 7.8, 0.3 M NaAC, 15% PEG4000 |
